These are the villains that are in Avengers Assemble.
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- M'Baku
- M.O.D.O.K.
- Mach-IV
- Madame Masque
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- Mangog
- Masters of Evil
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- Maximus
- Melter
- Meteorite
- Midgard Serpent
- Mindless Ones
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- Mojo
- Mole Man
- Molecule Kid (character)
- Molecule Man
- Morbius the Living Vampire
- Morgan Le Fay
- Mummies
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- Scientist Supreme
- Scorpio
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- Seeker
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- Shadow Council
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- Skrulls
- Skull-Net
- Skullbots
- Songbird
- Space Phantoms
- Speed Demon
- Sphinx Monster
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- Squadron Supreme
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- Stosh
- Super-Adaptoid
- Super-Adaptoids
- Super-Apes